Prototipo de descongelamiento de células eucariotas crio-preservadas mediante procesos de inducción de calor

Abstract

Cryopreservation is a method that subjects cells to very low temperatures in order to preserve them for long periods of time for later use. It is important to maintain high cell viability and functionality, both at the time of cryopreservation and thawing. Currently, the most commonly used method for cryopreserving cells is by using a serological bath at 37°C, in which the cryovial is immersed in water and subjected to manual agitation. However, it is known that this method has a potential risk of contamination, either due to dirty water, constant handling of the cryovial by the operator, overheating of the vial, among other factors. On the other hand, the conventional method does not offer traceability or automation of the cryopreservation process. This is why, in this project, an alternative to the conventional method was developed, proposing a conceptual prototype of eukaryotic cell thawing by means of an electromagnetic induction system, offering the operator the possibility of keeping the traceability of the samples, an automated, sterile, fast and reproducible system. This system achieves cell thawing in 2.5 to 2.7 minutes, provided that the setpoint temperature is set to 120°C. The results obtained guarantee that under these thawing parameters, it was possible to obtain a viability higher than 90%, and there were no significant differences compared to the traditional method.

Date of Award	2022
Original language	Spanish (Colombia)
Awarding Institution	Universidad Autónoma de Bucaramanga Ingeniería Biomédica
Supervisor	Víctor Alfonso Solarte David (Supervisor) & Silvia Milena Becerra Bayona (Supervisor)