Evaluación del efecto del cloruro de calcio y gluconato de calcio en la activación de plaquetas humanas

Abstract

Platelet activation is a frequent procedure in the area of regenerative medicine. Despite having a variety of protocols for platelet activation, these processes lack of adequate standardization where similar concentrations and release kinetics of growth factors (GF) can be obtained. Therefore, there is a need to evaluate different alternatives that lead to optimal platelet activation by implementing different methods, such as the use of activating agents derived from calcium. In this study, platelet activation was performed under different conditions; specifically, resuspending the platelet pellet in Platelet-poor plasma (PPP) and phosphate buffered saline (PBS) with the use of calcium chloride ('CaCl2') and calcium gluconate at concentrations of 10, 20 and 40 mM in time periods of 0, 5, 10, 15, 20, 30 minutes and 24 hours. Platelet activation induced by these two agents was evaluated by flow cytometry studying surface platelet markers, while the FC release was determined by spectrophotometry for the identification of total proteins (PT). Results obtained from platelet activation revealed that samples resuspended in PBS showed better activation percentages than those resuspended in PPP; Similarly, the samples activated with calcium gluconate presented higher percentages of platelet activation compared to 'CaCl2'. Regarding the release of FC, it was observed that in the group of samples resuspended in PPP, and activated by calcium gluconate reached a greater release than 'CaCl2' in each of the time periods, being the concentration of 40 mM the one that led to the highest release. This allows us to conclude that calcium gluconate reaches to have a better potential in activating human platelets than 'Cal2'.

Date of Award	25 Jan 2022
Original language	Spanish (Colombia)
Awarding Institution	Universidad Autónoma de Bucaramanga Ingeniería Biomédica
Supervisor	Silvia Milena Becerra Bayona (Supervisor) & Víctor Alfonso Solarte David (Supervisor)