TY - JOUR
T1 - Systemic changes following carrageenan-induced paw inflammation in rats
AU - Vazquez, E.
AU - Navarro, M.
AU - Salazar, Y.
AU - Crespo, G.
AU - Bruges, G.
AU - Osorio, C.
AU - Tortorici, V.
AU - Vanegas, H.
AU - López, Mercedes
N1 - Publisher Copyright:
© 2015, Springer Basel.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Objective and design: Carrageenan-induced paw edema has been described as a local and acute inflammatory process. In fact, little is known about the time course and systemic changes following a carrageenan injection. In this study, we examine the systemic changes that follow carrageenan injection in the paw. Methods: Acute inflammation was produced by subplantar injection of carrageenan in a hind paw of Sprague–Dawley rats. Saline was used in control rats. Paw volume was measured with a plethysmometer. The hot plate latency test was used to quantify antinociception. C-reactive protein (CRP) levels were measured with a sandwich enzyme immunoassay. Fibrinogen concentration was measured using the gravimetric method. Lung morphometric analysis was performed using an image processing package. Lungs and paws were also examined for tissue factor (TF) and proinflammatory cytokines expression by immunohistochemistry. Results: We found diverse systemic changes including increased levels of acute phase proteins, such as CRP and fibrinogen, and a lung inflammatory process characterized by lung edema, fibrin deposition, and leukocyte infiltration. An elevated expression of TF, IL-6, IL-1β, and TNFα, was observed in paw and lung tissue sections by immunohistochemical methods. Conclusion: This study provides new evidence that a local carrageenan injection induces a systemic response.
AB - Objective and design: Carrageenan-induced paw edema has been described as a local and acute inflammatory process. In fact, little is known about the time course and systemic changes following a carrageenan injection. In this study, we examine the systemic changes that follow carrageenan injection in the paw. Methods: Acute inflammation was produced by subplantar injection of carrageenan in a hind paw of Sprague–Dawley rats. Saline was used in control rats. Paw volume was measured with a plethysmometer. The hot plate latency test was used to quantify antinociception. C-reactive protein (CRP) levels were measured with a sandwich enzyme immunoassay. Fibrinogen concentration was measured using the gravimetric method. Lung morphometric analysis was performed using an image processing package. Lungs and paws were also examined for tissue factor (TF) and proinflammatory cytokines expression by immunohistochemistry. Results: We found diverse systemic changes including increased levels of acute phase proteins, such as CRP and fibrinogen, and a lung inflammatory process characterized by lung edema, fibrin deposition, and leukocyte infiltration. An elevated expression of TF, IL-6, IL-1β, and TNFα, was observed in paw and lung tissue sections by immunohistochemical methods. Conclusion: This study provides new evidence that a local carrageenan injection induces a systemic response.
KW - Carrageenan
KW - Lung inflammation
KW - Paw model
KW - Proinflammatory cytokines
KW - Tissue factor expression
UR - http://www.scopus.com/inward/record.url?scp=84939992389&partnerID=8YFLogxK
U2 - 10.1007/s00011-015-0814-0
DO - 10.1007/s00011-015-0814-0
M3 - Artículo Científico
C2 - 25772383
AN - SCOPUS:84939992389
SN - 1023-3830
VL - 64
SP - 333
EP - 342
JO - Inflammation Research
JF - Inflammation Research
IS - 5
ER -