TY - JOUR
T1 - Sequence diversity and evolution of multigene families in Trypanosoma cruzi
AU - Cerqueira, Gustavo C.
AU - Bartholomeu, Daniella C.
AU - DaRocha, Wanderson D.
AU - Hou, Lihua
AU - Freitas-Silva, Danielle M.
AU - Machado, Carlos Renato
AU - El-Sayed, Najib M.
AU - Teixeira, Santuza M.R.
N1 - Funding Information:
Supported by grants from CNPq and FAPEMIG, Brazil. G.C. Cerqueira doctorate fellowship is sponsored by CAPES, Brazil. The research of S.M.R. Teixeira was supported by an International Research Scholars Grant from the Howard Hughes Medical Institute. The research of N.E.S. was supported by a NIH grant (AI45038). We are grateful to Dr. Egler Chiari and Andrea M. Macedo, from Universidade Federal de Minas Gerais, Brazil, Dr. Bianca Zingales from Universidade de São Paulo, Brazil, and Dr. Samuel Goldenberg from the Instituto de Biologia Molecular do Parana, Brazil, for kindly providing parasite cultures or DNA from T. cruzi strains and to Dr. Scott Westenberger, from the University of California at Los Angeles, US, for discussions and helpful comments. We are also grateful to various administrative and technical personnel from The Institute for Genomic Research (TIGR), where part of this work was performed.
PY - 2008/1
Y1 - 2008/1
N2 - Several copies of genes belonging to three multigene families present in the genome of Trypanosoma cruzi were sequenced and comparatively analyzed across six different strains of the parasite belonging to the T. cruzi I lineage (Colombiana, Silvio X10 and Dm28c), the T. cruzi II lineage (Esmeraldo and JG) and a hybrid strain (CL Brener). For all three gene families analyzed, our results support the division in T. cruzi I and II lineages. Furthermore, in agreement with its hybrid nature, sequences derived from the CL Brener clone clustered together with T. cruzi II sequences as well as with a third group of sequences. Paralogous sequences encoding Amastin, an amastigote surface glycoprotein and TcAG48, an antigenic RNA binding protein, which are clustered in the parasite genome, present higher intragenomic variability in T. cruzi II and CL Brener strains, when compared to T. cruzi I strains. Paralogous sequences derived from the TcADC gene family, which encode various isoforms of adenylyl cyclases and are dispersed throughout the T. cruzi genome, exhibit similar degree of variability in all strains, except in the CL Brener strain, in which the sequences were more divergent. Several factors including mutation rates and gene conversion mechanisms, acting differently within the T. cruzi population, may contribute to create such distinct levels of sequence diversity in multigene families that are clustered in the T. cruzi genome.
AB - Several copies of genes belonging to three multigene families present in the genome of Trypanosoma cruzi were sequenced and comparatively analyzed across six different strains of the parasite belonging to the T. cruzi I lineage (Colombiana, Silvio X10 and Dm28c), the T. cruzi II lineage (Esmeraldo and JG) and a hybrid strain (CL Brener). For all three gene families analyzed, our results support the division in T. cruzi I and II lineages. Furthermore, in agreement with its hybrid nature, sequences derived from the CL Brener clone clustered together with T. cruzi II sequences as well as with a third group of sequences. Paralogous sequences encoding Amastin, an amastigote surface glycoprotein and TcAG48, an antigenic RNA binding protein, which are clustered in the parasite genome, present higher intragenomic variability in T. cruzi II and CL Brener strains, when compared to T. cruzi I strains. Paralogous sequences derived from the TcADC gene family, which encode various isoforms of adenylyl cyclases and are dispersed throughout the T. cruzi genome, exhibit similar degree of variability in all strains, except in the CL Brener strain, in which the sequences were more divergent. Several factors including mutation rates and gene conversion mechanisms, acting differently within the T. cruzi population, may contribute to create such distinct levels of sequence diversity in multigene families that are clustered in the T. cruzi genome.
KW - Amastin
KW - Gene conversion
KW - Genetic diversity
KW - Multigene families
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=36348960649&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2007.10.002
DO - 10.1016/j.molbiopara.2007.10.002
M3 - Artículo Científico
C2 - 18023889
AN - SCOPUS:36348960649
SN - 0166-6851
VL - 157
SP - 65
EP - 72
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1
ER -