Schistosoma mansoni: The IMP4 gene is involved in DNA repair/tolerance after treatment with alkylating agent methyl methane sulfonate

Carolina Furtado, Carlos Gustavo Regis-da-Silva, Danielle Gomes Passos-Silva, Glória Regina Franco, Andréa Mara Macedo, Sérgio Danilo Junho Pena, Carlos Renato Machado

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Using a functional complementation strategy, we have isolated a Schistosoma mansoni cDNA that complemented Escherichia coli mutant strains which are defective in the DNA base excision repair pathway. This cDNA partially complemented the MMS-sensitive phenotype of these strains. The sequence of the isolated cDNA was homologous to genes involved in the RNA metabolism pathway, especially ScIMP4 of Saccharomyces cerevisiae. To establish whether the S. mansoni cDNA clone could complement yeast ScIMP4-defective mutants, we constructed a yeast haploid strain that coded for a truncated Imp4p protein. This mutant strain was treated with different DNA damaging agents, but showed only MMS sensitivity. The functional homology between the ScIMP4 gene and the cDNA from S. mansoni was verified by partial complementation of the mutant yeast with the worm's gene. This gene appears to be involved in DNA repair and RNA metabolism in both S. mansoni and S. cerevisiae.

Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalExperimental Parasitology
Volume116
Issue number1
DOIs
StatePublished - May 2007
Externally publishedYes

Keywords

  • 6-methyl guanine methyl transferase
  • base excision repair
  • BER
  • cDNA
  • complementary DNA
  • deoxyribonucleic acid
  • DNA
  • DNA repair
  • IMP4
  • LB
  • Luria-Bertani
  • methyl methane sulfonate
  • MGMT
  • MMS
  • open reading frame
  • ORF
  • PCR
  • PEG
  • polyethylene glycol
  • polymerase chain reaction
  • ribonucleic acid
  • ribonucleic protein
  • ribosomal RNA
  • RNA
  • RNA metabolism
  • RNP
  • rRNA
  • Schistosoma mansoni
  • single-stranded DNA
  • small nucleolar RNA
  • small nucleolar RNP
  • snoRNA
  • snoRNP
  • ssDNA
  • ultraviolet
  • UV
  • yeast extract/peptone/dextrose
  • yeast nitrogen base
  • YNB
  • YPD

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