Safety of the intravenous administration of neurotensin-polyplex nanoparticles in BALB/c mice

Maria E. Hernandez, Jesus D. Rembao, Daniel Hernandez-Baltazar, Rosa A. Castillo-Rodriguez, Victor M. Tellez-Lopez, Yazmin M. Flores-Martinez, Carlos E. Orozco-Barrios, Hector A. Rubio, Aurora Sánchez-García, Jose Ayala-Davila, Martha L. Arango-Rodriguez, Lenin Pavón, Teresa Mejia-Castillo, Patricia Forgez, Daniel Martinez-Fong

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Neurotensin (NTS)-polyplex is a gene nanocarrier that has potential nanomedicine-based applications for the treatment of Parkinson's disease and cancers of cells expressing NTS receptor type 1. We assessed the acute inflammatory response to NTS-polyplex carrying a reporter gene in BALB/c mice. The intravenous injection of NTS-polyplex caused the specific expression of the reporter gene in gastrointestinal cells. Six hours after an intravenous injection of propidium iodide labeled-NTS-polyplex, fluorescent spots were located in the cells of the organs with a mononuclear phagocyte system, suggesting NTS-polyplex clearance. In contrast to lipopolysaccharide and carbon tetrachloride, NTS-polyplex did not increase the serum levels of tumor necrosis factor alpha, interleukin (IL)-1β, IL-6, bilirubin, aspartate transaminase, and alanine transaminase. NTS-polyplex increased the levels of serum amyloid A and alkaline phosphatase, but these levels normalized after 24 h. Compared to carrageenan, the local injection of NTS-polyplex did not produce inflammation. Our results support the safety of NTS-polyplex. From the Clinical Editor: This study focuses on the safety of neurotensin (NTS)-polyplex, a gene nanocarrier that has potential in the treatment of Parkinson's disease and cancers of cells expressing NTS receptor type 1. NTS polyplex demonstrates a better safety profile compared with carrageenan, lipopolysaccharide, and carbon tetrachloride in a murine model.

Original languageEnglish
Pages (from-to)745-754
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume10
Issue number4
DOIs
StatePublished - May 2014
Externally publishedYes

Keywords

  • Biosafety
  • Cytotoxicity
  • Nanomaterials
  • Pro-inflammatory cytokines

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