Rapid electrochemical detection of staphylococcus aureus based on screen-printed gold electrodes modified with cysteine and guinea grass (Panicum maximum) peroxidase

Paula Guarín; Jonatan Cristancho; John J. Castillo

doi:10.18257/RACCEFYN.1019

Rapid electrochemical detection of staphylococcus aureus based on screen-printed gold electrodes modified with cysteine and guinea grass (Panicum maximum) peroxidase

Paula Guarín, Jonatan Cristancho, John J. Castillo

Research output: Articles / Notes › Scientific Article › peer-review

5 Scopus citations

Abstract

We present a novel electrochemical method for a rapid analytical detection of Staphylococcus aureus in culture and spiked milk samples through hydrogen peroxide (H₂O₂) consumption on a screen-printed gold electrode (SPGE) modified with cysteine and peroxidase from Guinea grass leaves (GGP). This peroxidase (POD) had a specific activity of 470 U mg^-1 and it was immobilized on an SPGE surface previously modified with cysteine. Cyclic voltammograms of gold electrodes modified with peroxidase and cysteine in the presence of potassium ferrocyanide as a redox probe demonstrated an increase of approximately 5% in the current compared to the bare gold electrode. The SPGE modified electrode exhibited a good electrocatalytic response towards H₂O₂ reduction. We added a constant H₂O₂ concentration of 1x10^-3 M to the culture medium and measured the decrease in the H₂O₂ current at -780 mV consumed by catalase from S. aureus. Our modified electrode proved to sense S. aureus in a range concentration between 3x10² and 3x10⁸ CFU/mL^-1 with a detection limit of 102 CFU/mL^-1, detection time of about ~20 min, and a sensitivity of 0.020

Translated title of the contribution	Método electroquímico para la detección rápida y de staphylococcus aureus en cultivos por electrodos serigrafiados de oro modificados con cisteína y peroxidasa de pasto guinea (Panicum máximum)
Original language	English
Pages (from-to)	835-844
Number of pages	10
Journal	Revista de la Academia Colombiana de Ciencias Exactas, Fisicas y Naturales
Volume	44
Issue number	172
DOIs	https://doi.org/10.18257/RACCEFYN.1019
State	Published - Sep 2020
Externally published	Yes

Keywords

Electrochemical detection
Guinea grass peroxidase
Screen-printed electrodes
Staphylococcus aureus

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10.18257/RACCEFYN.1019

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title = "Rapid electrochemical detection of staphylococcus aureus based on screen-printed gold electrodes modified with cysteine and guinea grass (Panicum maximum) peroxidase",

abstract = "We present a novel electrochemical method for a rapid analytical detection of Staphylococcus aureus in culture and spiked milk samples through hydrogen peroxide (H2O2) consumption on a screen-printed gold electrode (SPGE) modified with cysteine and peroxidase from Guinea grass leaves (GGP). This peroxidase (POD) had a specific activity of 470 U mg-1 and it was immobilized on an SPGE surface previously modified with cysteine. Cyclic voltammograms of gold electrodes modified with peroxidase and cysteine in the presence of potassium ferrocyanide as a redox probe demonstrated an increase of approximately 5% in the current compared to the bare gold electrode. The SPGE modified electrode exhibited a good electrocatalytic response towards H2O2 reduction. We added a constant H2O2 concentration of 1x10-3 M to the culture medium and measured the decrease in the H2O2 current at -780 mV consumed by catalase from S. aureus. Our modified electrode proved to sense S. aureus in a range concentration between 3x102 and 3x108 CFU/mL-1 with a detection limit of 102 CFU/mL-1, detection time of about ~20 min, and a sensitivity of 0.020",

keywords = "Electrochemical detection, Guinea grass peroxidase, Screen-printed electrodes, Staphylococcus aureus",

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Rapid electrochemical detection of staphylococcus aureus based on screen-printed gold electrodes modified with cysteine and guinea grass (Panicum maximum) peroxidase. / Guarín, Paula; Cristancho, Jonatan; Castillo, John J.
In: Revista de la Academia Colombiana de Ciencias Exactas, Fisicas y Naturales, Vol. 44, No. 172, 09.2020, p. 835-844.

Research output: Articles / Notes › Scientific Article › peer-review

TY - JOUR

T1 - Rapid electrochemical detection of staphylococcus aureus based on screen-printed gold electrodes modified with cysteine and guinea grass (Panicum maximum) peroxidase

AU - Guarín, Paula

AU - Cristancho, Jonatan

AU - Castillo, John J.

PY - 2020/9

Y1 - 2020/9

N2 - We present a novel electrochemical method for a rapid analytical detection of Staphylococcus aureus in culture and spiked milk samples through hydrogen peroxide (H2O2) consumption on a screen-printed gold electrode (SPGE) modified with cysteine and peroxidase from Guinea grass leaves (GGP). This peroxidase (POD) had a specific activity of 470 U mg-1 and it was immobilized on an SPGE surface previously modified with cysteine. Cyclic voltammograms of gold electrodes modified with peroxidase and cysteine in the presence of potassium ferrocyanide as a redox probe demonstrated an increase of approximately 5% in the current compared to the bare gold electrode. The SPGE modified electrode exhibited a good electrocatalytic response towards H2O2 reduction. We added a constant H2O2 concentration of 1x10-3 M to the culture medium and measured the decrease in the H2O2 current at -780 mV consumed by catalase from S. aureus. Our modified electrode proved to sense S. aureus in a range concentration between 3x102 and 3x108 CFU/mL-1 with a detection limit of 102 CFU/mL-1, detection time of about ~20 min, and a sensitivity of 0.020

AB - We present a novel electrochemical method for a rapid analytical detection of Staphylococcus aureus in culture and spiked milk samples through hydrogen peroxide (H2O2) consumption on a screen-printed gold electrode (SPGE) modified with cysteine and peroxidase from Guinea grass leaves (GGP). This peroxidase (POD) had a specific activity of 470 U mg-1 and it was immobilized on an SPGE surface previously modified with cysteine. Cyclic voltammograms of gold electrodes modified with peroxidase and cysteine in the presence of potassium ferrocyanide as a redox probe demonstrated an increase of approximately 5% in the current compared to the bare gold electrode. The SPGE modified electrode exhibited a good electrocatalytic response towards H2O2 reduction. We added a constant H2O2 concentration of 1x10-3 M to the culture medium and measured the decrease in the H2O2 current at -780 mV consumed by catalase from S. aureus. Our modified electrode proved to sense S. aureus in a range concentration between 3x102 and 3x108 CFU/mL-1 with a detection limit of 102 CFU/mL-1, detection time of about ~20 min, and a sensitivity of 0.020

KW - Electrochemical detection

KW - Guinea grass peroxidase

KW - Screen-printed electrodes

KW - Staphylococcus aureus

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ER -

Rapid electrochemical detection of staphylococcus aureus based on screen-printed gold electrodes modified with cysteine and guinea grass (Panicum maximum) peroxidase

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