Polymorphisms in the IL4 and IL4RA genes in Colombian patients with rheumatoid arthritis

Olga Moreno, Clara Isabel González, Diego Luis Saaibi, William Otero, Reynaldo Badillo, Javier Martín, Gerardo Ramírez

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Objective. Rheumatoid arthritis (RA) is considered a Th1-driven disease. Interleukin 4 (IL-4) binds to its receptor, promoting Th2 differentiation and limiting Th1 responses, but its role in the patho-genesis of RA is conflicting. We analyzed 2 polymorphisms of the IL4 gene and 4 polymorphisms of the IL4RA gene in patients with RA and in a control population, as well as rheumatoid factor (RF) seropositivity, titers of RF, and history of replacement joint surgery among patients with RA. Methods. The study population consisted of 102 patients with RA and 102 matched healthy controls. Genotyping of IL4 -590, 1L4RA +148, +1124, +1218, and +1902 was determined by restriction fragment length polymorphism-polymerase chain reaction (PCR) and sequence-specific primer-PCR. IL4 variable number tandem repeat polymorphism was determined by direct amplification. Results. The IL4-590TT genotype was significantly more frequent in patients with RA than in controls (p = 0.018, OR 3.34, 95% CI 1.08-11.04). The IL4RA +148A allele was significantly associated with the presence of RF (p = 0.0019, OR 2.55, 95% CI 1.55-4.86) and a history of articular joint replacement (p = 0.024, OR 2.08, 95% CI 1.04-4.18). The IL4RA +1902G allele was more frequently seen in patients with RA and high RF titers (p = 0.00067, OR 4, 95% CI 1.64-9.93). Conclusion. Highly complex pathways lead to the development of RA and may not be similar in all patients. Our findings of higher frequency of IL4 and 1L4RA genotypes and alleles with RA, presence of RF, RF titers, and history of articular joint replacement support the polygenic expression of RA and the likely role of IL-4 in influencing its initiation and development.

Original languageEnglish
Pages (from-to)36-42
Number of pages7
JournalJournal of Rheumatology
Volume34
Issue number1
StatePublished - Jan 2007
Externally publishedYes

Keywords

  • Genetic predisposition
  • Interleukin 4
  • Rheumatoid arthritis

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