Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy

Lídia M. Andrade; Jony M. Geraldo; Osvaldo X. Gonçalves; Miguel T.T. Leite; Anderson M. Catarina; Melissa M. Guimarães; Adriana F.P. Leme; Sami Yokoo; Carlos R. Machado; Matheus A. Rajão; Sandhra M. Carvalho; Dawidson A. Gomes; Carla J. Aguiar; Elaine M. Souza-Fagundes; Carlos L. Zani; Rodrigo R. Resende; Olindo A. Martins-Filho; M. Fátima Leite

doi:10.4172/1948-5956.1000127

Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy

Lídia M. Andrade
, Jony M. Geraldo
, Osvaldo X. Gonçalves
, Miguel T.T. Leite
, Anderson M. Catarina
, Melissa M. Guimarães
, Adriana F.P. Leme
, Sami Yokoo
, Carlos R. Machado
, Matheus A. Rajão
, Sandhra M. Carvalho
, Dawidson A. Gomes
, Carla J. Aguiar
, Elaine M. Souza-Fagundes
, Carlos L. Zani
, Rodrigo R. Resende
, Olindo A. Martins-Filho
, M. Fátima Leite

Research output: Articles / Notes › Scientific Article › peer-review

16 Scopus citations

Abstract

Background: Calcium (Ca ²⁺) signaling within the nucleus is known to play a crucial role in cell proliferation. The aim of this study was to investigate whether nuclear Ca ²⁺ buffering could improve the antitumor effect of X-rays therapy on Human Squamous Cell Carcinoma (HSCC). Methods: For these purpose, we developed an experimental protocol that simulated clinical radiotherapy and prevented bystander effects of irradiation. HSCC, A431 cell line, was submitted to 10Gy cumulative X-rays therapy alone (XR _Cd10Gy) or in association with the strategy that selectively buffer nuclear Ca ²⁺ (Ca ²⁺ⁿ) signaling. Results: Upon Ca ²⁺ⁿ buffering, A431 cell proliferation rate decreased significantly as compared to control. Cell cycle analysis showed that association of Ca ²⁺ⁿ buffering with XR _Cd 10Gy increased the percentage of A431 cells at G ₂/M and did not increase nuclear/mitochondrial DNA damages. Nonetheless, Ca ²⁺ⁿ buffering prevented the increase of the radioresistance-related biomarker ADAM-17 expression and EGFR activation induced by irradiation. Furthermore, the association therapy almost completely abolished cell survival fraction even using approximately half of the X-rays cumulative dose. Conclusions: Nuclear Ca ²⁺ buffering sensitizes human squamous cell carcinoma to X-rays irradiation treatment.

Original language	English
Pages (from-to)	131-139
Number of pages	9
Journal	Journal of Cancer Science and Therapy
Volume	4
Issue number	5
DOIs	https://doi.org/10.4172/1948-5956.1000127
State	Published - 2012
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

A431 cells
Head and neck tumor
Human squamous cell carcinoma
Nuclear calcium buffering
X-rays irradiation

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10.4172/1948-5956.1000127

Cite this

Andrade, L. M., Geraldo, J. M., Gonçalves, O. X., Leite, M. T. T., Catarina, A. M., Guimarães, M. M., Leme, A. F. P., Yokoo, S., Machado, C. R., Rajão, M. A., Carvalho, S. M., Gomes, D. A., Aguiar, C. J., Souza-Fagundes, E. M., Zani, C. L., Resende, R. R., Martins-Filho, O. A., & Leite, M. F. (2012). Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy. Journal of Cancer Science and Therapy, 4(5), 131-139. https://doi.org/10.4172/1948-5956.1000127

@article{9e874bf4b8284d0287a4573ce5991436,

title = "Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy",

abstract = "Background: Calcium (Ca 2+) signaling within the nucleus is known to play a crucial role in cell proliferation. The aim of this study was to investigate whether nuclear Ca 2+ buffering could improve the antitumor effect of X-rays therapy on Human Squamous Cell Carcinoma (HSCC). Methods: For these purpose, we developed an experimental protocol that simulated clinical radiotherapy and prevented bystander effects of irradiation. HSCC, A431 cell line, was submitted to 10Gy cumulative X-rays therapy alone (XR Cd10Gy) or in association with the strategy that selectively buffer nuclear Ca 2+ (Ca 2+n) signaling. Results: Upon Ca 2+n buffering, A431 cell proliferation rate decreased significantly as compared to control. Cell cycle analysis showed that association of Ca 2+n buffering with XR Cd 10Gy increased the percentage of A431 cells at G 2/M and did not increase nuclear/mitochondrial DNA damages. Nonetheless, Ca 2+n buffering prevented the increase of the radioresistance-related biomarker ADAM-17 expression and EGFR activation induced by irradiation. Furthermore, the association therapy almost completely abolished cell survival fraction even using approximately half of the X-rays cumulative dose. Conclusions: Nuclear Ca 2+ buffering sensitizes human squamous cell carcinoma to X-rays irradiation treatment.",

keywords = "A431 cells, Head and neck tumor, Human squamous cell carcinoma, Nuclear calcium buffering, X-rays irradiation",

author = "Andrade, \{L{\'i}dia M.\} and Geraldo, \{Jony M.\} and Gon{\c c}alves, \{Osvaldo X.\} and Leite, \{Miguel T.T.\} and Catarina, \{Anderson M.\} and Guimar{\~a}es, \{Melissa M.\} and Leme, \{Adriana F.P.\} and Sami Yokoo and Machado, \{Carlos R.\} and Raj{\~a}o, \{Matheus A.\} and Carvalho, \{Sandhra M.\} and Gomes, \{Dawidson A.\} and Aguiar, \{Carla J.\} and Souza-Fagundes, \{Elaine M.\} and Zani, \{Carlos L.\} and Resende, \{Rodrigo R.\} and Martins-Filho, \{Olindo A.\} and Leite, \{M. F{\'a}tima\}",

year = "2012",

doi = "10.4172/1948-5956.1000127",

language = "Ingl{\'e}s",

volume = "4",

pages = "131--139",

journal = "Journal of Cancer Science and Therapy",

issn = "1948-5956",

publisher = "OMICS Publishing Group",

number = "5",

}

Andrade, LM, Geraldo, JM, Gonçalves, OX, Leite, MTT, Catarina, AM, Guimarães, MM, Leme, AFP, Yokoo, S, Machado, CR, Rajão, MA, Carvalho, SM, Gomes, DA, Aguiar, CJ, Souza-Fagundes, EM, Zani, CL, Resende, RR, Martins-Filho, OA & Leite, MF 2012, 'Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy', Journal of Cancer Science and Therapy, vol. 4, no. 5, pp. 131-139. https://doi.org/10.4172/1948-5956.1000127

TY - JOUR

T1 - Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy

AU - Andrade, Lídia M.

AU - Geraldo, Jony M.

AU - Gonçalves, Osvaldo X.

AU - Leite, Miguel T.T.

AU - Catarina, Anderson M.

AU - Guimarães, Melissa M.

AU - Leme, Adriana F.P.

AU - Yokoo, Sami

AU - Machado, Carlos R.

AU - Rajão, Matheus A.

AU - Carvalho, Sandhra M.

AU - Gomes, Dawidson A.

AU - Aguiar, Carla J.

AU - Souza-Fagundes, Elaine M.

AU - Zani, Carlos L.

AU - Resende, Rodrigo R.

AU - Martins-Filho, Olindo A.

AU - Leite, M. Fátima

PY - 2012

Y1 - 2012

N2 - Background: Calcium (Ca 2+) signaling within the nucleus is known to play a crucial role in cell proliferation. The aim of this study was to investigate whether nuclear Ca 2+ buffering could improve the antitumor effect of X-rays therapy on Human Squamous Cell Carcinoma (HSCC). Methods: For these purpose, we developed an experimental protocol that simulated clinical radiotherapy and prevented bystander effects of irradiation. HSCC, A431 cell line, was submitted to 10Gy cumulative X-rays therapy alone (XR Cd10Gy) or in association with the strategy that selectively buffer nuclear Ca 2+ (Ca 2+n) signaling. Results: Upon Ca 2+n buffering, A431 cell proliferation rate decreased significantly as compared to control. Cell cycle analysis showed that association of Ca 2+n buffering with XR Cd 10Gy increased the percentage of A431 cells at G 2/M and did not increase nuclear/mitochondrial DNA damages. Nonetheless, Ca 2+n buffering prevented the increase of the radioresistance-related biomarker ADAM-17 expression and EGFR activation induced by irradiation. Furthermore, the association therapy almost completely abolished cell survival fraction even using approximately half of the X-rays cumulative dose. Conclusions: Nuclear Ca 2+ buffering sensitizes human squamous cell carcinoma to X-rays irradiation treatment.

AB - Background: Calcium (Ca 2+) signaling within the nucleus is known to play a crucial role in cell proliferation. The aim of this study was to investigate whether nuclear Ca 2+ buffering could improve the antitumor effect of X-rays therapy on Human Squamous Cell Carcinoma (HSCC). Methods: For these purpose, we developed an experimental protocol that simulated clinical radiotherapy and prevented bystander effects of irradiation. HSCC, A431 cell line, was submitted to 10Gy cumulative X-rays therapy alone (XR Cd10Gy) or in association with the strategy that selectively buffer nuclear Ca 2+ (Ca 2+n) signaling. Results: Upon Ca 2+n buffering, A431 cell proliferation rate decreased significantly as compared to control. Cell cycle analysis showed that association of Ca 2+n buffering with XR Cd 10Gy increased the percentage of A431 cells at G 2/M and did not increase nuclear/mitochondrial DNA damages. Nonetheless, Ca 2+n buffering prevented the increase of the radioresistance-related biomarker ADAM-17 expression and EGFR activation induced by irradiation. Furthermore, the association therapy almost completely abolished cell survival fraction even using approximately half of the X-rays cumulative dose. Conclusions: Nuclear Ca 2+ buffering sensitizes human squamous cell carcinoma to X-rays irradiation treatment.

KW - A431 cells

KW - Head and neck tumor

KW - Human squamous cell carcinoma

KW - Nuclear calcium buffering

KW - X-rays irradiation

UR - https://www.scopus.com/pages/publications/84863227112

U2 - 10.4172/1948-5956.1000127

DO - 10.4172/1948-5956.1000127

M3 - Artículo Científico

AN - SCOPUS:84863227112

SN - 1948-5956

VL - 4

SP - 131

EP - 139

JO - Journal of Cancer Science and Therapy

JF - Journal of Cancer Science and Therapy

IS - 5

ER -

Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy

Abstract

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Keywords

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