NT-polyplex: A new tool for therapeutic gene delivery to neuroblastoma tumors

H. A. Rubio-Zapata, J. D. Rembao-Bojorquez, M. L. Arango-Rodriguez, S. Dupouy, P. Forgez, D. Martinez-Fong

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Neurotensin (NT)-polyplex is a nonviral system for the targeted gene delivery to cells that express and internalize the high-affinity NT receptor (NTSR1). In hemiparkinsonian rats, we previously demonstrated the morphological and functional recovery from dopaminergic neurodegeneration using the NT-polyplex as a vehicle to transfect a neurotrophic gene. The main objective of this work was to demonstrate the feasibility of NT-polyplex to transfect reporter or therapeutic genes into neuroblastoma tumors through the blood stream or by intratumoral injection. N1E-115 cells known to express NTSR1 were allografted into athymic mice to generate the neuroblastoma tumor model. Both routes of administration allowed the NT-polyplex to reach and transfect tumoral cells. A low transgene expression was also detected in intestinal tract cells only after the injection into the blood stream. The transfection of the thymidine kinase (HSVTK) suicide gene followed by ganciclovir (GCV) treatment decreased the size and weight of neuroblastoma tumors by 30-50% and increased apoptosis compared to controls. This study shows the potential of the NT-polyplex as specific gene-transfer system for NTSR1 cancer cells.

Original languageEnglish
Pages (from-to)573-584
Number of pages12
JournalCancer Gene Therapy
Volume16
Issue number7
DOIs
StatePublished - Jul 2009
Externally publishedYes

Keywords

  • Apoptosis
  • Endocytosis
  • Ganciclovir
  • HSVTK
  • Neurotensin receptor
  • Transfection

Fingerprint

Dive into the research topics of 'NT-polyplex: A new tool for therapeutic gene delivery to neuroblastoma tumors'. Together they form a unique fingerprint.

Cite this