TY - JOUR
T1 - Melatonin attenuates acute renal failure and oxidative stress induced by mercuric chloride in rats
AU - Nava, Mayerly
AU - Romero, Freddy
AU - Quiroz, Yasmir
AU - Parra, Gustavo
AU - Bonet, Lizette
AU - Rodríguez-Iturbe, Bernardo
PY - 2000
Y1 - 2000
N2 - We evaluated the effect of melatonin (Mel), a potent scavenger of reactive oxygen species, in the course of HgCl2-induced acute renal failure. Rats received by gastric gavage 1 mg/kg of Mel (n = 21) or vehicle (n = 21), 30 min before the subcutaneous injection of HgCl2 (2.5 mg/kg). Rats were killed at 24, 48, and 72 h, and plasma creatinine (S(cr)), renal histology, proliferative activity, apoptosis, and superoxide-producing cells were studied. We also determined the renal content of malondialdehyde (MDA) and glutathione (GSH) and the activities of glutathione peroxidase and catalase. Mel pretreatment (Mel plasma levels of 3.40 ± 3.15 μg/ml at the time of HgCl2 injection) prevented the increment in S(cr) and reduced tubular necrosis from 41.0 ± 10.5 to 4.2 ± 5.1% of proximal tubules (P < 0.01). Apoptosis and postnecrotic proliferative activity were twice more intense in the group untreated with Mel. Increment in renal content of MDA and decrease in GSH resulting from HgCl2 toxicity were prevented by Mel. Mel also induced an important reduction in superoxide-positive cells. In contrast to the beneficial effects of pretreatment with Mel, the administration of Mel in conjunction with HgCl2 had no effect on the oxidative damage and did not prevent nephrotoxicity. We conclude that the beneficial effects of pharmacological doses of Mel are due to its antioxidant properties.
AB - We evaluated the effect of melatonin (Mel), a potent scavenger of reactive oxygen species, in the course of HgCl2-induced acute renal failure. Rats received by gastric gavage 1 mg/kg of Mel (n = 21) or vehicle (n = 21), 30 min before the subcutaneous injection of HgCl2 (2.5 mg/kg). Rats were killed at 24, 48, and 72 h, and plasma creatinine (S(cr)), renal histology, proliferative activity, apoptosis, and superoxide-producing cells were studied. We also determined the renal content of malondialdehyde (MDA) and glutathione (GSH) and the activities of glutathione peroxidase and catalase. Mel pretreatment (Mel plasma levels of 3.40 ± 3.15 μg/ml at the time of HgCl2 injection) prevented the increment in S(cr) and reduced tubular necrosis from 41.0 ± 10.5 to 4.2 ± 5.1% of proximal tubules (P < 0.01). Apoptosis and postnecrotic proliferative activity were twice more intense in the group untreated with Mel. Increment in renal content of MDA and decrease in GSH resulting from HgCl2 toxicity were prevented by Mel. Mel also induced an important reduction in superoxide-positive cells. In contrast to the beneficial effects of pretreatment with Mel, the administration of Mel in conjunction with HgCl2 had no effect on the oxidative damage and did not prevent nephrotoxicity. We conclude that the beneficial effects of pharmacological doses of Mel are due to its antioxidant properties.
KW - Nephrotoxic acute renal failure
UR - http://www.scopus.com/inward/record.url?scp=0033680803&partnerID=8YFLogxK
U2 - 10.1152/ajprenal.2000.279.5.f910
DO - 10.1152/ajprenal.2000.279.5.f910
M3 - Artículo Científico
C2 - 11053052
AN - SCOPUS:0033680803
SN - 1931-857X
VL - 279
SP - F910-F918
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 5 48-5
ER -