In vitro comparison of cytocompatibility between fibroin and polypropylene biomaterials

Manuel Hernando Franco Arias; Alejandro Arboleda Carvajal; Liliana Valladares

In vitro comparison of cytocompatibility between fibroin and polypropylene biomaterials

Manuel Hernando Franco Arias, Alejandro Arboleda Carvajal, Liliana Valladares

Research output: Articles / Notes › Scientific Article › peer-review

Abstract

This study evaluates the growth of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture with these biomaterials, the number of cells/sample was obtained by metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol Bromide, MTT assay, using direct and indirect evidence of cytotoxicity. For direct and indirect testing of cytotoxicity in fibroin and polypropylene materials, a statistical difference (p<0.005) was found in the average number of living cells in favor to the fibroin samples, regardless of the cytotoxicity test (i.e., direct or indirect), suggesting that the fibroin material allows for greater cell growth.
The in vitro methods showed that fibroin material has better cell behavior in terms of viability, compared with
polypropylene.

Translated title of the contribution	Comparación in vitro de la citocompatibilidad entre los biomateriales fibroína y polipropileno
Original language	English
Pages (from-to)	97-107
Journal	Revista Facultad de Ingeniería
State	Published - May 2017

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title = "In vitro comparison of cytocompatibility between fibroin and polypropylene biomaterials",

abstract = "This study evaluates the growth of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture with these biomaterials, the number of cells/sample was obtained by metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol Bromide, MTT assay, using direct and indirect evidence of cytotoxicity. For direct and indirect testing of cytotoxicity in fibroin and polypropylene materials, a statistical difference (p<0.005) was found in the average number of living cells in favor to the fibroin samples, regardless of the cytotoxicity test (i.e., direct or indirect), suggesting that the fibroin material allows for greater cell growth.The in vitro methods showed that fibroin material has better cell behavior in terms of viability, compared withpolypropylene.",

author = "{Franco Arias}, {Manuel Hernando} and {Arboleda Carvajal}, Alejandro and Liliana Valladares",

year = "2017",

month = may,

language = "Ingl{\'e}s",

pages = "97--107",

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T1 - In vitro comparison of cytocompatibility between fibroin and polypropylene biomaterials

AU - Franco Arias, Manuel Hernando

AU - Arboleda Carvajal, Alejandro

AU - Valladares, Liliana

PY - 2017/5

Y1 - 2017/5

N2 - This study evaluates the growth of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture with these biomaterials, the number of cells/sample was obtained by metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol Bromide, MTT assay, using direct and indirect evidence of cytotoxicity. For direct and indirect testing of cytotoxicity in fibroin and polypropylene materials, a statistical difference (p<0.005) was found in the average number of living cells in favor to the fibroin samples, regardless of the cytotoxicity test (i.e., direct or indirect), suggesting that the fibroin material allows for greater cell growth.The in vitro methods showed that fibroin material has better cell behavior in terms of viability, compared withpolypropylene.

AB - This study evaluates the growth of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture with these biomaterials, the number of cells/sample was obtained by metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol Bromide, MTT assay, using direct and indirect evidence of cytotoxicity. For direct and indirect testing of cytotoxicity in fibroin and polypropylene materials, a statistical difference (p<0.005) was found in the average number of living cells in favor to the fibroin samples, regardless of the cytotoxicity test (i.e., direct or indirect), suggesting that the fibroin material allows for greater cell growth.The in vitro methods showed that fibroin material has better cell behavior in terms of viability, compared withpolypropylene.

M3 - Artículo Científico

SN - 2357-5328

SP - 97

EP - 107

JO - Revista Facultad de Ingeniería

JF - Revista Facultad de Ingeniería

ER -