TY - JOUR
T1 - In vitro and in silico analyses of Lippia alba (Verbenaceae) essential oil as an inhibitor of dengue virus and platelet activation
AU - Silva-Trujillo, Lina
AU - Ocazionez, Raquel E.
AU - Quintero-Rueda, Elizabeth
AU - Stashenko, Elena E.
AU - Rondón-Villarreal, Paola
AU - Solarte-David, Victor A.
N1 - Publisher Copyright:
© 2024 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2024
Y1 - 2024
N2 - Dengue virus (DENV) causes millions of infections each year, and there are currently no approved antivirals. Essential oils could serve as potential candidates for developing plant-based dengue treatments. Lippia alba (Mill.) N.E. Brown essential oil (LAEO) was selected to investigate the mode of antiviral action and its effect on activated platelets. Binding affinities of 20 LAEO compounds and platelet proteins were investigated through docking analyses. LAEO showed a potent virucidal effect (IC50, 2.1 to 5.1 μg/mL) against all DENV serotypes. LAEO reduced P-selectin (from 61% to 18%) and increased survival (from 71% to 97%) in DENV-2- and DENV NS1-stimulated human platelets. Sesquiterpene hydrocarbons showed the highest binding affinities (from −7.3 to −8.0 kcal/mol) with platelet innate immune receptors such as TLR2/1, TLR4/MD-2 and αIIbβ3 integrin. The data provide a first step towards defining the potential of LAEO as a candidate for developing phytotherapeutics for dengue.
AB - Dengue virus (DENV) causes millions of infections each year, and there are currently no approved antivirals. Essential oils could serve as potential candidates for developing plant-based dengue treatments. Lippia alba (Mill.) N.E. Brown essential oil (LAEO) was selected to investigate the mode of antiviral action and its effect on activated platelets. Binding affinities of 20 LAEO compounds and platelet proteins were investigated through docking analyses. LAEO showed a potent virucidal effect (IC50, 2.1 to 5.1 μg/mL) against all DENV serotypes. LAEO reduced P-selectin (from 61% to 18%) and increased survival (from 71% to 97%) in DENV-2- and DENV NS1-stimulated human platelets. Sesquiterpene hydrocarbons showed the highest binding affinities (from −7.3 to −8.0 kcal/mol) with platelet innate immune receptors such as TLR2/1, TLR4/MD-2 and αIIbβ3 integrin. The data provide a first step towards defining the potential of LAEO as a candidate for developing phytotherapeutics for dengue.
KW - Essential oil
KW - Lippia alba
KW - dengue
KW - dengue virus
KW - platelet activation
KW - thrombocytopenia
UR - http://www.scopus.com/inward/record.url?scp=85198756901&partnerID=8YFLogxK
U2 - 10.1080/10412905.2024.2371825
DO - 10.1080/10412905.2024.2371825
M3 - Artículo Científico
AN - SCOPUS:85198756901
SN - 1041-2905
VL - 36
SP - 469
EP - 480
JO - Journal of Essential Oil Research
JF - Journal of Essential Oil Research
IS - 5
ER -
