@article{6b67a8f9d67e46cc8fe7b2bc0300ed1a,
title = "Genetic analyses of trypanosoma cruzi isolates from naturally infected triatomines and humans in northeastern Brazil",
abstract = "Trypanosoma cruzi genetic diversity was investigated in 25 isolates (vectors and humans) from the semiarid zone of the State of Rio Grande do Norte, Brazil. Molecular markers (3' region of the 24Sα rRNA; mitochondrial cytochrome oxidase subunit 2 (COII) gene; spliced leader intergenic region (SL-IR) gene; allelic size microsatellite polymorphism) identified 56% TcIII (100% Panstrongylus lutzi; 50% Triatoma brasiliensis); 40% TcII (91.7% humans; 50% T. brasiliensis) and 4% TcI (human). Microsatellite analysis revealed monoclonal and heterozygous patterns on one or more microsatellite loci in 64% of T. cruzi isolates (92.3% triatomines; 33.3% humans) and 36% putative polyclonal populations (66.7% humans; 7.7% triatomines) by loci SCLE10, SCLE11, TcTAT20, TcAAAT6, all belonging to TcII. Identical T. cruzi polyclonal profiles (88.9%) were detected, mostly from humans. The adaptative natural plasticity of TcII and TcIII and their potential for maintaining human infection in T. brasiliensis were confirmed. Intraspecific and phylogenetic T. cruzi diversity in the sylvatic and domestic transmission cycles in this specific region will provide exclusive control strategies.",
keywords = "Chagas disease infection, Genomic markers, Panstrongylus lutzi, T. cruzi III, Triatoma brasiliensis, Trypanosoma cruzi",
author = "C{\^a}mara, {A. C.J.} and Varela-Freire, {A. A.} and Valadares, {H. M.S.} and Macedo, {A. M.} and D'{\'A}vila, {D. A.} and Machado, {C. R.} and E. Lages-Silva and E. Chiari and Galv{\~a}o, {L. M.C.}",
note = "Funding Information: This work received financial support from the Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq/Edital Universal/2006), Edital MCT/CNPq/MS-SCTIE-DECIT 25/2006-Estudo de Doen{\c c}as Negligenciadas and fellowships from the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento do Pessoal do Ensino Superior—Plano Institucional de Capacita{\c c}{\~a}o Docente (doctoral) and CNPq (Visitor Researcher). The authors are grateful to the Esta{\c c}{\~a}o Ecol{\'o}gica do Serid{\'o} (ESEC), Instituto Chico Mendes de Conserva{\c c}{\~a}o da Biodiversidade (ICMBIO), Secretaria de Sa{\'u}de do Estado Rio Grande do Norte, Brasil, municipal authorities and local health teams for their unconditional support for the development of this study, and Edson Santana from the Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Orlando Carlos Magno and Afonso Costa Viana from the Departamento de Parasitologia, Universidade Federal de Minas Gerais for their technical assistance. The authors also wish to thank Philip S.P. Badiz for his critical reading and revision of the manuscript.",
year = "2010",
month = sep,
doi = "10.1016/j.actatropica.2010.03.003",
language = "Ingl{\'e}s",
volume = "115",
pages = "205--211",
journal = "Acta Tropica",
issn = "0001-706X",
publisher = "Elsevier B.V.",
number = "3",
}
