Expression pattern of protease activated receptors in lymphoid cells

Mercedes L. López; Natalia Soriano-Sarabia; Gustavo Bruges; María Elena Marquez; Klaus T. Preissner; M. Lienhard Schmitz; Holger Hackstein

doi:10.1016/j.cellimm.2014.02.004

Expression pattern of protease activated receptors in lymphoid cells

Mercedes L. López, Natalia Soriano-Sarabia, Gustavo Bruges, María Elena Marquez, Klaus T. Preissner, M. Lienhard Schmitz, Holger Hackstein

Research output: Articles / Notes › Scientific Article › peer-review

23 Scopus citations

Abstract

Protease-activated receptors (PARs) are a subfamily of four G-protein-coupled receptors mediating multiple functions. PARs expression was studied in subpopulations of human lymphocytes. Our results indicate that natural killer cells expressed mRNA for PAR₁, PAR₂ and PAR₃, CD4+ T cells expressed PAR₁ and PAR₂, while γδ and CD8+ T cells only expressed PAR₁. PAR₄ was absent at mRNA level and B cells did not express any PAR. Analyses of the cell surface PARs expression by flow cytometry were consistent with the mRNA data and also between different donors. PAR₁ is the most abundant member of the PAR family present in lymphocytes.

Original language	English
Pages (from-to)	47-52
Number of pages	6
Journal	Cellular Immunology
Volume	288
Issue number	1-2
DOIs	https://doi.org/10.1016/j.cellimm.2014.02.004
State	Published - Mar 2014
Externally published	Yes

Keywords

B cells
FACS
Lymphocytes
Protease-activated receptors

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10.1016/j.cellimm.2014.02.004

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@article{f46867cb27774aaa921c2813cfadc175,

title = "Expression pattern of protease activated receptors in lymphoid cells",

abstract = "Protease-activated receptors (PARs) are a subfamily of four G-protein-coupled receptors mediating multiple functions. PARs expression was studied in subpopulations of human lymphocytes. Our results indicate that natural killer cells expressed mRNA for PAR1, PAR2 and PAR3, CD4+ T cells expressed PAR1 and PAR2, while γδ and CD8+ T cells only expressed PAR1. PAR4 was absent at mRNA level and B cells did not express any PAR. Analyses of the cell surface PARs expression by flow cytometry were consistent with the mRNA data and also between different donors. PAR1 is the most abundant member of the PAR family present in lymphocytes.",

keywords = "B cells, FACS, Lymphocytes, Protease-activated receptors",

author = "L{\'o}pez, {Mercedes L.} and Natalia Soriano-Sarabia and Gustavo Bruges and Marquez, {Mar{\'i}a Elena} and Preissner, {Klaus T.} and Schmitz, {M. Lienhard} and Holger Hackstein",

note = "Funding Information: This work was performed during the tenure of a Fellowship for Experienced Researchers from the Alexander von Humboldt Foundation to Mercedes L{\'o}pez. Part of the work was supported by the Excellence cluster “Cardiopulmonary System” (ECCPS) , Deutsche Forschungsgemeinschaft (Bonn, Germany) .",

year = "2014",

month = mar,

doi = "10.1016/j.cellimm.2014.02.004",

language = "Ingl{\'e}s",

volume = "288",

pages = "47--52",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "1-2",

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TY - JOUR

T1 - Expression pattern of protease activated receptors in lymphoid cells

AU - López, Mercedes L.

AU - Soriano-Sarabia, Natalia

AU - Bruges, Gustavo

AU - Marquez, María Elena

AU - Preissner, Klaus T.

AU - Schmitz, M. Lienhard

AU - Hackstein, Holger

N1 - Funding Information: This work was performed during the tenure of a Fellowship for Experienced Researchers from the Alexander von Humboldt Foundation to Mercedes López. Part of the work was supported by the Excellence cluster “Cardiopulmonary System” (ECCPS) , Deutsche Forschungsgemeinschaft (Bonn, Germany) .

PY - 2014/3

Y1 - 2014/3

N2 - Protease-activated receptors (PARs) are a subfamily of four G-protein-coupled receptors mediating multiple functions. PARs expression was studied in subpopulations of human lymphocytes. Our results indicate that natural killer cells expressed mRNA for PAR1, PAR2 and PAR3, CD4+ T cells expressed PAR1 and PAR2, while γδ and CD8+ T cells only expressed PAR1. PAR4 was absent at mRNA level and B cells did not express any PAR. Analyses of the cell surface PARs expression by flow cytometry were consistent with the mRNA data and also between different donors. PAR1 is the most abundant member of the PAR family present in lymphocytes.

AB - Protease-activated receptors (PARs) are a subfamily of four G-protein-coupled receptors mediating multiple functions. PARs expression was studied in subpopulations of human lymphocytes. Our results indicate that natural killer cells expressed mRNA for PAR1, PAR2 and PAR3, CD4+ T cells expressed PAR1 and PAR2, while γδ and CD8+ T cells only expressed PAR1. PAR4 was absent at mRNA level and B cells did not express any PAR. Analyses of the cell surface PARs expression by flow cytometry were consistent with the mRNA data and also between different donors. PAR1 is the most abundant member of the PAR family present in lymphocytes.

KW - B cells

KW - FACS

KW - Lymphocytes

KW - Protease-activated receptors

UR - http://www.scopus.com/inward/record.url?scp=84896332535&partnerID=8YFLogxK

U2 - 10.1016/j.cellimm.2014.02.004

DO - 10.1016/j.cellimm.2014.02.004

M3 - Artículo Científico

C2 - 24637088

AN - SCOPUS:84896332535

SN - 0008-8749

VL - 288

SP - 47

EP - 52

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1-2

ER -

Expression pattern of protease activated receptors in lymphoid cells

Abstract

Keywords

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