Escherichia coli as a model system to study DNA repair genes of eukaryotic organisms

Luiz Augusto-Pinto, Carlos Gustavo Regis Da Silva, Débora De Oliveira Lopes, Alice Machado-Silva, Carlos Renato Machado

Research output: Articles / NotesScientific Articlepeer-review

14 Scopus citations

Abstract

The bacteria Escherichia coli has been widely employed in studies of eukaryotic DNA repair genes. Several eukaryotic genes have been cloned by functional complementation of mutant lineages of E. coli. We examined the similarities and differences among bacterial and eukaryotic DNA repair systems. Based on these data, we examined tools used for gene cloning and functional studies of DNA repair in eukaryotes, using this bacterial system as a model.

Original languageEnglish
Pages (from-to)77-91
Number of pages15
JournalGenetics and Molecular Research
Volume2
Issue number1
StatePublished - 2003
Externally publishedYes

Keywords

  • DNA repair
  • Escherichia coli
  • Functional complementation

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