TY - JOUR
T1 - Electroanalytical determination of catechol by a biosensor based on laccase from Aspergillus oryzae immobilized on gold screen-printed electrodes
AU - Caballero, S. J.
AU - Guerrero, M. A.
AU - Vargas, L. Y.
AU - Ortiz, C. C.
AU - Castillo, J. J.
AU - Gutiérrez, J. A.
AU - Blanco, S.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2018/12/10
Y1 - 2018/12/10
N2 - In the present work, the detection of catechol (CC) was studied by using a biosensor of gold screen-printed electrode functionalized with laccase and cysteine as a linker. Cyclic voltammetry (CVs) studies were performed in order to determine the stability range of the biosensor and the redox processes present on the surface of the modified screen-printed gold electrodes (SPGE). CVs studies showed a variation in the response of the electrodes, finding one oxidation signal at 400 mV in presence of catechol, corresponding to the bio-electrocatalytic oxidation-of catechol. The chronoamperometric response of the sensor to different concentration of catechol was measured applying 500 mV, obtaining a linear response of the current in function of the catechol concentration. The linear range of the detection was 0.02-2.4 mM, with a sensibility of 3.93 μA μM -1 cm -2 and a detection limit of 1.2 μM with a response time lower than 5 s. The results obtained in this work open new possibilities for the development of portable electrochemical biosensors for quick and efficient detection of catechol in industrial wastewater.
AB - In the present work, the detection of catechol (CC) was studied by using a biosensor of gold screen-printed electrode functionalized with laccase and cysteine as a linker. Cyclic voltammetry (CVs) studies were performed in order to determine the stability range of the biosensor and the redox processes present on the surface of the modified screen-printed gold electrodes (SPGE). CVs studies showed a variation in the response of the electrodes, finding one oxidation signal at 400 mV in presence of catechol, corresponding to the bio-electrocatalytic oxidation-of catechol. The chronoamperometric response of the sensor to different concentration of catechol was measured applying 500 mV, obtaining a linear response of the current in function of the catechol concentration. The linear range of the detection was 0.02-2.4 mM, with a sensibility of 3.93 μA μM -1 cm -2 and a detection limit of 1.2 μM with a response time lower than 5 s. The results obtained in this work open new possibilities for the development of portable electrochemical biosensors for quick and efficient detection of catechol in industrial wastewater.
UR - http://www.scopus.com/inward/record.url?scp=85058691691&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1119/1/012009
DO - 10.1088/1742-6596/1119/1/012009
M3 - Artículo de la conferencia
AN - SCOPUS:85058691691
SN - 1742-6588
VL - 1119
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012009
T2 - 3rd Colombian Congress of Electrochemistry of the Colombian Association of Electrochemistry, CCEQ 2018
Y2 - 2 October 2018 through 5 October 2018
ER -