Concordancia de dos pruebas serológicas para el diagnóstico de la enfermedad de Chagas

Ana E. Farfán-García; Yeny Z. Castellanos-Domínguez; Katherine P. Luna-Marín; Víctor M. Angulo-Silva

Concordancia de dos pruebas serológicas para el diagnóstico de la enfermedad de Chagas

Translated title of the contribution: Concordance of two serological tests for the diagnosis of Chagas disease

Ana E. Farfán-García
, Yeny Z. Castellanos-Domínguez
, Katherine P. Luna-Marín
, Víctor M. Angulo-Silva

Research output: Articles / Notes › Scientific Article › peer-review

3 Scopus citations

Abstract

OBJECTIVE: Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.

METHODOLOGY: A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.

RESULTS: The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.

CONCLUSIONS: The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.

Translated title of the contribution	Concordance of two serological tests for the diagnosis of Chagas disease
Original language	Spanish
Pages (from-to)	208-219
Number of pages	12
Journal	Revista de Salud Publica
Volume	15
Issue number	2
State	Published - 1 Mar 2013
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Cite this

@article{8e453da83e724dc2949e96306d63d7dc,

title = "Concordancia de dos pruebas serol{\'o}gicas para el diagn{\'o}stico de la enfermedad de Chagas",

abstract = "OBJECTIVE: Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.METHODOLOGY: A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.RESULTS: The in-house ELISA it was an agreement of 0.99 (95 \% CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 \% were positive by ELISA and 10.9 \% by IIF.CONCLUSIONS: The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.",

author = "Farf{\'a}n-Garc{\'i}a, \{Ana E.\} and Castellanos-Dom{\'i}nguez, \{Yeny Z.\} and Luna-Mar{\'i}n, \{Katherine P.\} and Angulo-Silva, \{V{\'i}ctor M.\}",

year = "2013",

month = mar,

day = "1",

language = "Espa{\~n}ol",

volume = "15",

pages = "208--219",

journal = "Revista de Salud Publica",

issn = "0124-0064",

publisher = "Universidad Nacional de Colombia",

number = "2",

}

TY - JOUR

T1 - Concordancia de dos pruebas serológicas para el diagnóstico de la enfermedad de Chagas

AU - Farfán-García, Ana E.

AU - Castellanos-Domínguez, Yeny Z.

AU - Luna-Marín, Katherine P.

AU - Angulo-Silva, Víctor M.

PY - 2013/3/1

Y1 - 2013/3/1

N2 - OBJECTIVE: Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.METHODOLOGY: A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.RESULTS: The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.CONCLUSIONS: The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.

AB - OBJECTIVE: Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.METHODOLOGY: A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.RESULTS: The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.CONCLUSIONS: The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.

UR - https://www.scopus.com/pages/publications/84920944668

M3 - Artículo Científico

C2 - 24892663

AN - SCOPUS:84920944668

SN - 0124-0064

VL - 15

SP - 208

EP - 219

JO - Revista de Salud Publica

JF - Revista de Salud Publica

IS - 2

ER -

Concordancia de dos pruebas serológicas para el diagnóstico de la enfermedad de Chagas

Abstract

UN SDGs

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