Concordancia de dos pruebas serológicas para el diagnóstico de la enfermedad de Chagas

Translated title of the contribution: Agreement between two serological tests for the diagnosis of Chaga's disease

Ana E. Farfán-García, Yeny Z. Castellanos-Domínguez, Katherine P. Luna-Marín, Víctor M. Angulo-Silva

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: Establishing agreement between an in-house ELISA and an indirect immunofluorescence (IIF) assay for diagnosing infection caused byT. cruzi using blood eluates. Methodology: The study involved an evaluation of diagnostic technology and cross-sectional sampling of 650 people residing in an endemic area of Colombia. The area below the receiver operating characteristic (ROC) curve was determined and IIF was used as gold standard. The cut-off for the ELISA was established, as well as agreement between readings. Results: The in-house ELISA had 0.99 agreement (0.989 to 0.992 95%CI) between the two readings taken and ROC curve area was 0.9795. The ELISA test cut-off point was set at 0.5 absorbance; 16.6 % were positive by ELISA and 10.9 % by IIF. Conclusions: The in-house ELISA assay had good agreement compared to IIF; it would thus seem a good diagnostic choice for testing a target population living in remote areas.

Translated title of the contributionAgreement between two serological tests for the diagnosis of Chaga's disease
Original languageSpanish
Pages (from-to)192-202
Number of pages11
JournalRevista de Salud Publica
Volume15
Issue number2
StatePublished - 2013
Externally publishedYes

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