Agreement between two serological tests for the diagnosis of Chaga's disease

Ana E. Farfán-García; Yeny Z. Castellanos-Domínguez; Katherine P. Luna-Marín; Víctor M. Angulo-Silva

Agreement between two serological tests for the diagnosis of Chaga's disease

Translated title of the contribution: Agreement between two serological tests for the diagnosis of Chaga's disease

Ana E. Farfán-García, Yeny Z. Castellanos-Domínguez, Katherine P. Luna-Marín, Víctor M. Angulo-Silva

Research output: Articles / Notes › Scientific Article › peer-review

1 Scopus citations

Abstract

Objective: Establishing agreement between an in-house ELISA and an indirect immunofluorescence (IIF) assay for diagnosing infection caused byT. cruzi using blood eluates. Methodology: The study involved an evaluation of diagnostic technology and cross-sectional sampling of 650 people residing in an endemic area of Colombia. The area below the receiver operating characteristic (ROC) curve was determined and IIF was used as gold standard. The cut-off for the ELISA was established, as well as agreement between readings. Results: The in-house ELISA had 0.99 agreement (0.989 to 0.992 95%CI) between the two readings taken and ROC curve area was 0.9795. The ELISA test cut-off point was set at 0.5 absorbance; 16.6 % were positive by ELISA and 10.9 % by IIF. Conclusions: The in-house ELISA assay had good agreement compared to IIF; it would thus seem a good diagnostic choice for testing a target population living in remote areas.

Translated title of the contribution	Agreement between two serological tests for the diagnosis of Chaga's disease
Original language	English
Pages (from-to)	192-202
Number of pages	11
Journal	Revista de Salud Publica
Volume	15
Issue number	2
State	Published - 2013
Externally published	Yes

Keywords

Chaga's disease
Diagnosis
Serology
Trypanosomacruzi

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Agreement between two serological tests for the diagnosis of Chaga's disease",

abstract = "Objective: Establishing agreement between an in-house ELISA and an indirect immunofluorescence (IIF) assay for diagnosing infection caused byT. cruzi using blood eluates. Methodology: The study involved an evaluation of diagnostic technology and cross-sectional sampling of 650 people residing in an endemic area of Colombia. The area below the receiver operating characteristic (ROC) curve was determined and IIF was used as gold standard. The cut-off for the ELISA was established, as well as agreement between readings. Results: The in-house ELISA had 0.99 agreement (0.989 to 0.992 95%CI) between the two readings taken and ROC curve area was 0.9795. The ELISA test cut-off point was set at 0.5 absorbance; 16.6 % were positive by ELISA and 10.9 % by IIF. Conclusions: The in-house ELISA assay had good agreement compared to IIF; it would thus seem a good diagnostic choice for testing a target population living in remote areas.",

keywords = "Chaga's disease, Diagnosis, Serology, Trypanosomacruzi",

author = "Farf{\'a}n-Garc{\'i}a, {Ana E.} and Castellanos-Dom{\'i}nguez, {Yeny Z.} and Luna-Mar{\'i}n, {Katherine P.} and Angulo-Silva, {V{\'i}ctor M.}",

year = "2013",

language = "Ingl{\'e}s",

volume = "15",

pages = "192--202",

journal = "Revista de Salud Publica",

issn = "0124-0064",

publisher = "Universidad Nacional de Colombia",

number = "2",

}

TY - JOUR

T1 - Agreement between two serological tests for the diagnosis of Chaga's disease

AU - Farfán-García, Ana E.

AU - Castellanos-Domínguez, Yeny Z.

AU - Luna-Marín, Katherine P.

AU - Angulo-Silva, Víctor M.

PY - 2013

Y1 - 2013

N2 - Objective: Establishing agreement between an in-house ELISA and an indirect immunofluorescence (IIF) assay for diagnosing infection caused byT. cruzi using blood eluates. Methodology: The study involved an evaluation of diagnostic technology and cross-sectional sampling of 650 people residing in an endemic area of Colombia. The area below the receiver operating characteristic (ROC) curve was determined and IIF was used as gold standard. The cut-off for the ELISA was established, as well as agreement between readings. Results: The in-house ELISA had 0.99 agreement (0.989 to 0.992 95%CI) between the two readings taken and ROC curve area was 0.9795. The ELISA test cut-off point was set at 0.5 absorbance; 16.6 % were positive by ELISA and 10.9 % by IIF. Conclusions: The in-house ELISA assay had good agreement compared to IIF; it would thus seem a good diagnostic choice for testing a target population living in remote areas.

AB - Objective: Establishing agreement between an in-house ELISA and an indirect immunofluorescence (IIF) assay for diagnosing infection caused byT. cruzi using blood eluates. Methodology: The study involved an evaluation of diagnostic technology and cross-sectional sampling of 650 people residing in an endemic area of Colombia. The area below the receiver operating characteristic (ROC) curve was determined and IIF was used as gold standard. The cut-off for the ELISA was established, as well as agreement between readings. Results: The in-house ELISA had 0.99 agreement (0.989 to 0.992 95%CI) between the two readings taken and ROC curve area was 0.9795. The ELISA test cut-off point was set at 0.5 absorbance; 16.6 % were positive by ELISA and 10.9 % by IIF. Conclusions: The in-house ELISA assay had good agreement compared to IIF; it would thus seem a good diagnostic choice for testing a target population living in remote areas.

KW - Chaga's disease

KW - Diagnosis

KW - Serology

KW - Trypanosomacruzi

UR - http://www.scopus.com/inward/record.url?scp=84897540106&partnerID=8YFLogxK

M3 - Artículo Científico

AN - SCOPUS:84897540106

SN - 0124-0064

VL - 15

SP - 192

EP - 202

JO - Revista de Salud Publica

JF - Revista de Salud Publica

IS - 2

ER -

Agreement between two serological tests for the diagnosis of Chaga's disease

Abstract

Keywords

UN SDGs

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