Carácterísticas histológicas de piel cultivada in vitro

Translated title of the contribution: Histological characteristics of skin culture in vitro

Martha Ligia Arango Rodríguez, C. I. Chamorro, L. M. Restrepo, L. A. Correa, J. Henao

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In vitro skin culture have been used in the regeneration of skin wound (chronic ulcers and burns), and for genodermatosis treatment. In our country there is a high patient number with chronic ulcers and 2319 burned in a period of 10 years. Conventional treatment generates long hospitalization stays and high costs. We established culture conditions of keratinocytes and fibroblasts expansion, to generate a cutaneous substitute in order to offer other treatment options. Skin cells were obtained from organs donors and surgical surpluses procedures. Major success was achieved in primary cultures obtained from 40-year-old younger donors samples (65%), compared with older donors (33%). In one cutaneous substitute produced with these cells, was demonstrated that keratinocytes and fibroblasts, presented functional, structural and morphologic characteristics similar to normal skin. Cutaneous substitute besides preserve normal skin functional and structural characteristic, compared with other similar imported products, our cutaneous substitute, showed many advantages in terms of costs, manipulation and stability.

Translated title of the contributionHistological characteristics of skin culture in vitro
Original languageSpanish
Pages (from-to)190-200
Number of pages11
JournalRevista Argentina de Dermatologia
Volume90
Issue number4
StatePublished - Oct 2009
Externally publishedYes

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