Association of pre-eclampsia risk with maternal levels of folate, homocysteine and vitamin B12 in Colombia: A case-control study

Norma C. Serrano, Doris Cristina Quintero-Lesmes, Silvia Becerra-Bayona, Elizabeth Guio, Mónica Beltran, María C. Paez, Ricardo Ortiz, Wilmar Saldarriaga, Luis A. Diaz, Álvaro Monterrosa, Jezid Miranda, Clara M. Mesa, José E. Sanin, German Monsalve, Frank Dudbridge, Aroon D. Hingorani, Juan P. Casas

Research output: Articles / NotesScientific Articlepeer-review

35 Scopus citations

Abstract

Background Maternal serum concentrations of folate, homocysteine, and vitamin B12 have been associated with pre-eclampsia. Nevertheless, reported studies involve limited number of cases to reliably assess the nature of these associations. Our aim was to examine the relation of these three biomarkers with pre-eclampsia risk in a large Colombian population. Materials and methods Design: A case-control study. Setting: Cases of pre-eclampsia and healthy pregnant controls were recruited at the time of delivery from eight different Colombian cities between 2000 and 2012. Population or Sample: 2978 cases and 4096 controls were studied. Maternal serum concentrations of folate, homocysteine, and vitamin B12 were determined in 1148 (43.6%) cases and 1300 (31.7%) controls. Also, self-reported folic acid supplementation was recorded for 2563 (84%) cases and 3155 (84%) controls. Analysis: Adjusted odds ratios (OR) for pre-eclampsia were estimated for one standard deviation (1SD) increase in log-transformed biomarkers. Furthermore, we conducted analyses to compare women that reported taking folic acid supplementation for different periods during pregnancy. Main Outcomes Measures: Odds ratio for pre-eclampsia. Results After adjusting for potential confounders in logistic regression models, the OR for preeclampsia was 0.80 (95% CI: 0.72, 0.90) for 1SD increase in log-folate, 1.16 (95%CI: 1.05, 1.27) for 1SD increase in log-homocysteine, and 1.10 (95%CI: 0.99, 1.22) for 1SD increase in log-vitamin B12. No interactions among the biomarkers were identified. Women who self-reported consumption of folic acid (1 mg/day) throughout their pregnancy had an adjusted OR for pre-eclampsia of 0.86 (95%CI: 0.67, 1.09) compared to women that reported no consumption of folic acid at any point during pregnancy. Conclusions Maternal serum concentrations of folate were associated as a protective factor for preeclampsia while concentrations of homocysteine were associated as a risk factor. No association between maternal vitamin B12 concentrations and preeclampsia was found.

Original languageEnglish
Article numbere0208137
JournalPLoS ONE
Volume13
Issue number12
DOIs
StatePublished - Dec 2018

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