Actividad proapoptótica de la estaurosporina en cultivos axénicos de Trypanosoma evansi

Translated title of the contribution: Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi

Gustavo Bruges, Meyerling Betancourt, Mariana March, Evangelina Sanchez, Alfredo Mijares

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Trypanosoma evansi is a blood protozoan parasite of the genus Trypanosoma which is responsible for surra (Trypanosomosis) in domestic and wild animals. This study addressed apoptotic-like features in Trypanosoma evansi in vitro. The mechanism of parasite death was investigated using staurosporine as an inducing agent. We evaluated its effects through several cytoplasmic features of apoptosis, including cell shrinkage, phosphatidylserine exposure, maintenance of plasma membrane integrity, and mitochondrial trans-membrane potential. For access to these features we have used the flow cytometry and fluorescence microscopy with cultures in the stationary phase and adjusted to a density of 10 6 cells/mL. The apoptotic effect of staurosporine in T. evansi was evaluated at 20 nM final concentration. There was an increase of phosphatidylserine exposure, whereas mitochondrial potential was decreased. Moreover, no evidence of cell permeability increasing with staurosporine was observed in this study, suggesting the absence of a necrotic process. Additional studies are needed to elucidate the possible pathways associated with this form of cell death in this hemoparasite.

Translated title of the contributionApoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi
Original languageSpanish
Pages (from-to)103-108
Number of pages6
JournalRevista do Instituto de Medicina Tropical de Sao Paulo
Volume54
Issue number2
DOIs
StatePublished - Mar 2012
Externally publishedYes

Fingerprint

Dive into the research topics of 'Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi'. Together they form a unique fingerprint.

Cite this