A hydrogen peroxide biosensor based on the immobilization of the highly stable royal palm tree peroxidase (Roystonea regia) with chitosan and glutaraldehyde on screen-printed graphene electrodes

Erika N. Villamizar; Carlos A. Ríos; John J. Castillo

A hydrogen peroxide biosensor based on the immobilization of the highly stable royal palm tree peroxidase (Roystonea regia) with chitosan and glutaraldehyde on screen-printed graphene electrodes

Erika N. Villamizar, Carlos A. Ríos, John J. Castillo

Research output: Articles / Notes › Scientific Article › peer-review

6 Scopus citations

Abstract

This paper present the fabrication and electrochemical studies of a screen-printed graphene electrode (SPGE) modified with Royal palm tree peroxidase (RPTP) in combination with chitosan (CS) and cross-linking of glutaraldehyde (GA) for detection of hydrogen peroxide. Cyclic voltammograms in the presence of potassium ferrocyanide ([Fe(CN)₆]^3-/4-) as a redox species demonstrated an increasing of 50μA evidenced by the electron transfer process of SPGE modified with CS-GA-RPTP. The graphene modified electrode exhibits excellent electrocatalytic activity to the reduction of H₂O₂, with a linear response in the 100 μM to 5 mM concentration range and a detection limit of 87 μM. The new sensor based on the modification of graphene electrode with the high stable RPTP will provide a potential amperometric detection system for determination of H₂O₂ in real samples with some biomedical or environmental importance.

Original language	English
Pages (from-to)	135-140
Number of pages	6
Journal	Journal of the Mexican Chemical Society
Volume	60
Issue number	3
State	Published - 1 Jul 2016
Externally published	Yes

Keywords

Graphene
Hydrogen peroxide
Royal palm tree peroxidase
Sensor

Cite this

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title = "A hydrogen peroxide biosensor based on the immobilization of the highly stable royal palm tree peroxidase (Roystonea regia) with chitosan and glutaraldehyde on screen-printed graphene electrodes",

abstract = "This paper present the fabrication and electrochemical studies of a screen-printed graphene electrode (SPGE) modified with Royal palm tree peroxidase (RPTP) in combination with chitosan (CS) and cross-linking of glutaraldehyde (GA) for detection of hydrogen peroxide. Cyclic voltammograms in the presence of potassium ferrocyanide ([Fe(CN)6]3-/4-) as a redox species demonstrated an increasing of 50μA evidenced by the electron transfer process of SPGE modified with CS-GA-RPTP. The graphene modified electrode exhibits excellent electrocatalytic activity to the reduction of H2O2, with a linear response in the 100 μM to 5 mM concentration range and a detection limit of 87 μM. The new sensor based on the modification of graphene electrode with the high stable RPTP will provide a potential amperometric detection system for determination of H2O2 in real samples with some biomedical or environmental importance.",

keywords = "Graphene, Hydrogen peroxide, Royal palm tree peroxidase, Sensor",

author = "Villamizar, {Erika N.} and R{\'i}os, {Carlos A.} and Castillo, {John J.}",

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year = "2016",

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volume = "60",

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A hydrogen peroxide biosensor based on the immobilization of the highly stable royal palm tree peroxidase (Roystonea regia) with chitosan and glutaraldehyde on screen-printed graphene electrodes. / Villamizar, Erika N.; Ríos, Carlos A.; Castillo, John J.
In: Journal of the Mexican Chemical Society, Vol. 60, No. 3, 01.07.2016, p. 135-140.

Research output: Articles / Notes › Scientific Article › peer-review

TY - JOUR

T1 - A hydrogen peroxide biosensor based on the immobilization of the highly stable royal palm tree peroxidase (Roystonea regia) with chitosan and glutaraldehyde on screen-printed graphene electrodes

AU - Villamizar, Erika N.

AU - Ríos, Carlos A.

AU - Castillo, John J.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - This paper present the fabrication and electrochemical studies of a screen-printed graphene electrode (SPGE) modified with Royal palm tree peroxidase (RPTP) in combination with chitosan (CS) and cross-linking of glutaraldehyde (GA) for detection of hydrogen peroxide. Cyclic voltammograms in the presence of potassium ferrocyanide ([Fe(CN)6]3-/4-) as a redox species demonstrated an increasing of 50μA evidenced by the electron transfer process of SPGE modified with CS-GA-RPTP. The graphene modified electrode exhibits excellent electrocatalytic activity to the reduction of H2O2, with a linear response in the 100 μM to 5 mM concentration range and a detection limit of 87 μM. The new sensor based on the modification of graphene electrode with the high stable RPTP will provide a potential amperometric detection system for determination of H2O2 in real samples with some biomedical or environmental importance.

AB - This paper present the fabrication and electrochemical studies of a screen-printed graphene electrode (SPGE) modified with Royal palm tree peroxidase (RPTP) in combination with chitosan (CS) and cross-linking of glutaraldehyde (GA) for detection of hydrogen peroxide. Cyclic voltammograms in the presence of potassium ferrocyanide ([Fe(CN)6]3-/4-) as a redox species demonstrated an increasing of 50μA evidenced by the electron transfer process of SPGE modified with CS-GA-RPTP. The graphene modified electrode exhibits excellent electrocatalytic activity to the reduction of H2O2, with a linear response in the 100 μM to 5 mM concentration range and a detection limit of 87 μM. The new sensor based on the modification of graphene electrode with the high stable RPTP will provide a potential amperometric detection system for determination of H2O2 in real samples with some biomedical or environmental importance.

KW - Graphene

KW - Hydrogen peroxide

KW - Royal palm tree peroxidase

KW - Sensor

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M3 - Artículo Científico

AN - SCOPUS:84992735824

SN - 1870-249X

VL - 60

SP - 135

EP - 140

JO - Journal of the Mexican Chemical Society

JF - Journal of the Mexican Chemical Society

IS - 3

ER -

A hydrogen peroxide biosensor based on the immobilization of the highly stable royal palm tree peroxidase (Roystonea regia) with chitosan and glutaraldehyde on screen-printed graphene electrodes

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Keywords

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